Dawid WNUK, MSc

Cell Bank/ATMP laboratory 2.0.8
tel. (12) 664 61 89
e-mail: dawid.wnuk@uj.edu.pl
www: https://orcid.org/0000-0002-5807-2440

2014 – MSc in biology, spec. in cell biology, Faculty of Biology and Earth Sciences, Jagiellonian University
2017 – Management system specialist, Quality System Auditor (International Register of Certificated Auditors - IRCA), manager of systems: Quality Management, Environmental Management and Health and Safety Management (Lloyd's Register Quality Assurance) - Postgraduate studies: "Management and auditing", Institute of Economics, Finance and Management, Faculty of Management and Social Communication, Jagiellonian University

Scientific Internships and Awards »

  1. Academic-business internship (October 2015 – March 2016) of Jagiellonian Consortium for Biomolecular and Cellular Research Cell-Mol-Tech (KNOW) of the pro-quality grant of the National Scientific Lead Center in the Field of Biological Sciences. Elpharma
  2. Polish Ministry of Science and Higher Education Scholarship for outstanding achievements (biological sciences) 2017/2018
  3. Doctoral Scholarship ETIUDA7, Polish National Science Center (2019-2020)
  4. PhD Student OPUS9 Scholarship, Polish National Science Centre (2016-2019)
  5. J. Zurzycki Academic Scholarship for Publication Achievements in academic years: 2015/2016 and 2016/2017
  6. Pro-Quality, Jagiellonian University Rector's PhD Scholarship in academic year 2016/2017
  7. Bursary of Federation of European Biochemical Societies 2017; 42nd FEBS Congress 2017
  8. Bursary of Federation of European Biochemical Societies 2019; Lecture Course FEBS/EMBO „Molecular mechanisms of tissue injury, repair and fibrosis” Spetses, Greece

Scientific Interests »

  • Fibroblast to myofibroblast transition (FMT) during wound healing and in the development of fibrotic diseases and bronchial asthma
  • Receptor-dependent signalling pathways and transduction of biochemical signals in normal and cancer cells
  • Use of advanced therapy medical products (ATMP) to treat burns (autologous keratinocytes) and to treat knee degeneration (autologous keratinocytes)
  • Research on plant and lichenic compounds in the treatment of fibrotic diseases and neoplastic diseases (in the partnership with the Faculty of Pharmacy, Jagiellonian University)
  • Phenotypic heterogeneity of the tumor cell populations and their cytostatic resistance (search for effective anti-cancer and anti-tumor active agents)
  • Study of biological properties of newly-synthesized compounds as a potential drug carriers (in the cooperation with the Faculty of Chemistry, Jagiellonian University)
  • In vitro cultures of human bronchial cells (epithelium, fibroblasts) in 3D models: ALI and EMTU as a tool for intercellular interaction studies in respiratory disease models (in the cooperation with the Department of Pulmonology of the Jagiellonian University Hospital, Collegium Medicum)

Teaching »

Selected publications »

  1. Piska Kamil, Koczurkiewicz Paulina, Wnuk Dawid, Karnas Elżbieta, Bucki Adam, Wójcik-Pszczoła Katarzyna, Jamrozik Marek, Michalik Marta, Kołaczkowski Marcin, Pękala Elżbieta (2019) Synergistic anticancer activity of doxorubicin and piperlongumine on DU-145 prostate cancer cells - The involvement of carbonyl reductase 1 inhibition, Chemico Biological Interactions; https://doi.org/10.1016/j.cbi.2019.01.003
  2. Wiktor Anna, Sarna Michał, Wnuk Dawid, Sarna Tadeusz (2018) Lipofuscin-mediated photodynamic stress induces adverse changes in nanomechanical properties of retinal pigment epithelium cells, Scientific Reports, https://doi.org/10.1038/s41598-018-36322-2
  3. Kwolek Urszula; Nakai Keita; Pluta Anna; Zatorska Maria; Wnuk Dawid; Lasota Sławomir, Bednar Jan, Michalik Marta, Yusa Shin-ichi; Kepczynski Mariusz (2017) Polyion Complex Vesicles (PICsomes) from Strong Copolyelectrolytes, Colloids and Surfaces B: Biointerfaces, https://doi.org/10.1016/j.colsurfb.2017.07.042
  4. Paw Milena; Borek Izabela; Wnuk Dawid; Ryszawy Damian; Piwowarczyk Katarzyna; Kmiotek Katarzyna; Wojcik-Pszczola Katarzyna; Pierzchalska Malgorzata; Madeja Zbigniew; Sanak Marek; Blyszczuk Przemyslaw; Michalik Marta; Czyz Jaroslaw (2017) Connexin43 Controls the Myofibroblastic Differentiation of Bronchial Fibroblasts from Asthmatic Patients, American Journal of Respiratory Cell and Molecular Biology https://doi.org/10.1165/rcmb.2015-0255OC
  5. Galanty Agnieszka; Koczurkiewicz Paulina; Wnuk Dawid; Paw Milena; Karnas Elzbieta; Podolak Irma; Wegrzyn Michal; Borusiewicz Magdalena; Madeja Zbigniew; Czyz Jaroslaw; Michalik Marta; (2017) Usnic acid and atranorin exert selective cytostatic and anti-invasive effects on human prostate and melanoma cancer cells, Toxicology in Vitro, https://doi.org/10.1016/j.tiv.2017.01.008
  6. Sarna Michal; Olchawa Magdalena; Zadlo Andrzej; Wnuk Dawid; Sarna Tadeusz; (2017) The nanomechanical role of melanin granules in the retinal pigment epithelium, Nanomedicine: Nanotechnology, Biology and Medicine, https://doi.org/10.1016/j.nano.2016.11.020
  7. Koczurkiewicz Paulina; Kowolik Ewa; Podolak Irma; Wnuk Dawid; Piska Kamil; Labedz-Maslowska Anna; Wojcik-Pszczola Katarzyna; Pekala Elzbieta; Czyz Jaroslaw; Michalik Marta; (2016) Synergistic cytotoxic and anti-invasive effects of mitoxantrone and triterpene saponins from Lysimachia ciliata on human prostate cancer cells, Planta Medica, DOI: 10.1055/s-0042-117537
  8. Pilat Anna; Sarna Michal; Wnuk Dawid; Sarna Tadeusz; (2016) 178-Oxidative, Morphological and Mechanical Changes in ARPE-19 Cells Induced by Lipofuscin-Mediated Photic Stress, Free Radical Biology and Medicine, https://doi.org/10.1016/j.freeradbiomed.2016.10.219
  9. Wnuk Dawid; Kmiotek Katarzyna; Ryszawy Damian; Paw Milena; Michalik Marta; (2016) Inhibition of RhoA pathway decreases formin-related genes expression and TGF-β1-induced fibroblast to myofibroblast transition of bronchial fibroblasts derived from asthmatic patients, Respirology, 21, 213-213 (conference)
  10. Ryszawy Damian; Wnuk Dawid; Karnas Elzbieta; Paw Milena; Madeja Zbigniew; Michalik Marta; (2016) Endocytosis may be involved in asthma development by regulation of TGF-β-dependent human bronchial fibroblasts to miofibroblast transition, Respirology, 21, 213-213 (conference)
  11. Pilat Anna; Sarna Michal; Wnuk Dawid; Olchawa Magdalena; Sarna Tadeusz; (2016) Aging and antioxidants differentially modulate the effect of phagocytized lipofuscin granules on expression of antioxidant enzymes in ARPE-19 cells, Investigative Ophthalmology & Visual Science, 57, 12, 6044-6044 (conference)
  12. Wojcik-Pszczola Katarzyna; Hincza Kinga; Wnuk Dawid; Kadziolka Dominika; Koczurkiewicz Paulina; Sanak Marek; Madeja Zbigniew; Pekala Elzbieta; Michalik Marta; (2016) Pentoxifylline and its active metabolite lisofylline attenuate transforming growth factor β1-induced asthmatic bronchial fibroblast-to-myofibroblast transition, Acta Biochimica Polonica, https://doi.org/10.18388/abp.2016_1357
  13. Sarna Michal; Wojcik Katarzyna; Hermanowicz Pawel; Wnuk Dawid; Burda Kvetoslava; Sanak Marek; Czyz Jaroslaw; Michalik Marta; (2015) Undifferentiated bronchial fibroblasts derived from asthmatic patients display higher elastic modulus than their non-asthmatic counterparts, PLoS One, https://doi.org/10.1371/journal.pone.0116840