Automatic fluorescence microscope enabling, in addition to the applications described for the Leica DM IRE2 microscope, also fast ratiometric measurements and the use of FRET and TIRF techniques. In particular, the microscope has:
- equipment for fluorescence, contrast IMC and DIC
- LD lenses: 10x, 20x, 40x and oil: 40x, 63x, 100x
- EL 6000 fluorescent illuminator
- argon laser
- scanning table
- BL type incubator with CO2 control
- two digital cooled CCD cameras (12 bit Leica DFC360 FX and 14 bit Hamamatsu 9100-02 EM-CCD High Speed Set)
- AF7000 system, two-monitor with software for deconvolution, 3D imaging, signal colocation and FRET, FURA2 and TIRF analyzes
- anti-vibration table
- narrowband fluorescent filters (four sets: A4; L5; N3; Y5)
- three-band filter: UV, blue, green, with narrow-band emission filters: BP 465/20, BP 530/30 andBP 640/40 for IFW system
- equipment for FRET and FURA2 techniques and a system for quick image acquisition
- equipment for the TIRF technique